Coding

Part:BBa_K336052:Design

Designed by: Naoto Shimada   Group: iGEM10_Tokyo_Metropolitan   (2010-10-27)


bacterial cellulose synthase C


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3145
    Illegal PstI site found at 328
    Illegal PstI site found at 685
    Illegal PstI site found at 1243
    Illegal PstI site found at 1558
    Illegal PstI site found at 2503
    Illegal PstI site found at 2836
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3145
    Illegal PstI site found at 328
    Illegal PstI site found at 685
    Illegal PstI site found at 1243
    Illegal PstI site found at 1558
    Illegal PstI site found at 2503
    Illegal PstI site found at 2836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3145
    Illegal BglII site found at 1767
    Illegal BamHI site found at 2193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3145
    Illegal PstI site found at 328
    Illegal PstI site found at 685
    Illegal PstI site found at 1243
    Illegal PstI site found at 1558
    Illegal PstI site found at 2503
    Illegal PstI site found at 2836
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3145
    Illegal PstI site found at 328
    Illegal PstI site found at 685
    Illegal PstI site found at 1243
    Illegal PstI site found at 1558
    Illegal PstI site found at 2503
    Illegal PstI site found at 2836
    Illegal NgoMIV site found at 31
    Illegal NgoMIV site found at 488
    Illegal NgoMIV site found at 1666
    Illegal NgoMIV site found at 1725
    Illegal AgeI site found at 1216
    Illegal AgeI site found at 3412
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2752


Design Notes

only design


Source

this gene is derive from A.xylinum JCM7664 strain

References